Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.
نویسندگان
چکیده
The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. One of the predicted mobile elements is a previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing conjugative plasmids, one encoding a previously undescribed pheromone inhibitor. The apparent propensity for the incorporation of mobile elements probably contributed to the rapid acquisition and dissemination of drug resistance in the enterococci.
منابع مشابه
Evaluation of drug sensitivity of Entrococcus faecium and Entrococcus faecalis Strains and Detection of VanA/B Genes in Vancomycin Resistance Isolated by PCR Method
Enterococci, as the major cause of nosocomial infections, have been shown to have an increase in drug resistance during the past two decades. TheEntrococcalemergence of Vancomycin-resistant is very important nowadays. The purpose of this study was to determine the drug sensitivity of Enterococcus faecalis and Enterococcus faecium species against Vancomycin, Tetracycline, Gentamicin, Erythromyci...
متن کاملThe Inhibitory Effect of Lactobacillus rhamnosus GG on Vancomycin Resistant Enterococcus faecalis Colonization in Mouse
Background&Aims: Vancomycin Resistant Enterococci (VRE) are among the most common nosocomial pathogens worldwide. The intestinal tract provides a major source for transmission of these bacteria. Probiotics are living microorganisms that moderate use of them has inhibitory effect on intestinal colonization by enteric pathogens. We examined the effect of Lactobacillus rhamnosus GG (LGG) on inhibi...
متن کاملEvaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B
Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...
متن کاملAntibiogram analysis and tracking of the virulence-related genes in Enterococcus faecalis isolates
Background and Aim: Enterococcus species are opportunistic pathogens and their pathogenicity seems to be related to the presence of a number of pathogenicity genes. Since donkey’s milk is a new non-allergenic source of nutrition, this study was performed to assess the antibiogram and detection of pathogenicity genes in some Enterococcus faecalis isolates from donkey’s milk. Materials and Method...
متن کاملCharacterization of Enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production
In this study, 72 Enterococcus faecalis isolates originating from humans (n=39), dogs (n=26) and cats (n=7) were investigated for some virulence factors, some virulence genes, antibiotic resistance phenotypes, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) patterns and biofilm production. Of the isolates, 31 (43.1%) were positive for gelatinase, 11 (15.3%) for aggregati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Science
دوره 299 5615 شماره
صفحات -
تاریخ انتشار 2003